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Dahl Chase Folders

Collection of Bone Marrow

Reference Number: AA-00427





Collection of Bone Marrow Specimens

 

PURPOSE:

To ensure standardized collection of bone marrows, smear preparation, paper work and delivery to the appropriate departments (Northern Light Laboratory, ARUP, and Dahl-Chase Diagnostic Services Pathology and Flow Cytometry).

           

Test

Specimen

Forms and other

Send to

Pathology

Core in B Plus

Clot in B Plus

Smears on cardboard            tray

1.  Hematopathology requisition

2.  Demographic  sheet

3.  most recent CBC

Dahl-Chase (Webber West 5th floor)

Flow Cytometry

1-2cc in Na Heparin

Cytogenetics 

Add 1-2cc marrow in Na Heparin Invert several times.

1. Hematopathology requisition

2. ARUP Cytogenetics History Form

3. Demographic Sheet

FISH

Add 0.5-1cc marrow in Na Heparin Invert several times.

1. Hematopathology requisition 2. Demographic Sheet

Culture (bacterial, and fungal

1-1.5cc in Na Heparin

 Hematopathology requisition

Deliver immediately to ALI Lab (Webber West / 2ndfloor)

AFB

0.5-1cc cc in Na Heparin Tube

 Hematopathology requisition

Viral culture

EDTA Tube (lavender) or Na Citrate tube

Hematopathology requisition

JAK-2

3ml of bm in  EDTA

 Hematopathology requisition

Molecular PCR

1-3ml of bm in one EDTA (purple top)

 Hematopathology requisition

FLT3 PCR

3ml Na Heparin

Hematopathology requisition