Dahl Chase Folders

Anal HPV Screening Protocol

Reference Number: AA-00317

Anal HPV Screening Protocol


The “Anal Pap” is a screening tool used in at-risk populations to identify individuals who have premalignant cytologic changes in their anal epithelium.


1.       Moisten the Dacron swab with water, not lubricant.

2.       Insert Dacron swab* approximately 1.5 to 2 inches into the anal canal.* It is important to use Dacron and not a cotton swab, as cells tend to cling to cotton and do not release easily into cytology collection fluid.

3.       Once inserted deep enough into the anus (necessary in order to collect both rectal columnar and anal squamous cells), the swab should be pulled out, applying some pressure to the wall of the anus, rotating the swab in a spiral motion along the way.

4.       The collection device should be thoroughly rinsed and swirled in the SurePath vial.  After rinsing, the collection device may be discarded.

5.       Place the cap on the vial and tighten.

6.       Place the SurePath vial in a plastic biohazard bag and use zip lock to seal. Insert completed Non-Gyn Cytology Request Form in the outside pocket. 

Human Papilloma Virus (HPV) Testing may be ordered, in addition to routine cytology screening. However, HPV Testing on anal samples has not been validated or FDA approved. Therefore, the significance of these results is uncertain. A negative result does not exclude the possibility of an infection. If you wish to have the sample tested for HPV, please indicate on the requisition.

Materials and Reagents:

  • SurePath Vial
  • Dacron Swab - Moistened with Water
  • Non-Gyn Cytology Request Form
  • Biohazard Bag


Reference: ARUP Laboratories, 500 Chipeta Way, Salt Lake City, UT 84108