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Dahl Chase Folders

Neisseria Gonorrhoeae (NG) and Chlamydia Trachomatis (CT) Testing

Reference Number: AA-00420

SECTION EIGHT

Dahl-Chase Diagnostic Services

MOLECULAR TESTING

NEISSERIA GONORRHOEAE (NG) AND CHLAMYDIA TRACHOMATIS (CT) TESTING

 

Methodology

cobas® PCR

 

Principle

The cobas® CT/NG v2.0 Test for Chlamydia trachomatis and Neisseria gonorrhoeae is based on two major processes:  (1) automated sample preparation to obtain nucleic acids, including CT and NG DNA; (2) simultaneous PCR amplification of target DNA sequences using both CT and NG specific primer pairs and real-time detection of cleaved fluorescent-labeled CT and NG specific oligonucleotide detection probes.  An Internal Control, containing CT and NG DNA, is added to all samples prior to automated sample preparation and is amplified and detected simultaneously with each sample to monitor the entire process. These tests are performed together to confirm or exclude Chlamydia Trachomatis (CT) and/or Neisseria Gonorrhoeae (NG) infections.

 

Specimen Requirements

CT/NG testing is based on the use of a cobas® PCR Female swab kit.  These are available through Dahl-Chase purchasing department.

 

Specimen Collection

1.    Vaginal Collection -  Insert the first swab in to clean away any vaginal mucus and discard.  Insert the second swab about 5cm (2 inches) into the vaginal opening.  Gently turn the swab for about 30 seconds while rubbing the swab against the wall of the vagina.  Remove the swab carefully and place it directly into the tube, breaking the swab off so that it remains in the tube.  Cover and send to the lab with appropriate paperwork.

2.    Endocervical Collection - Insert the first swab into the endocervical canal to clean away any mucus and discard.  Insert the second swab into the endocervical canal.  Gently rotate the swab 5 times in one direction in the endocervical canal.  Do not over-rotate.  Carefully withdraw the swab, avoiding any contact with the vaginal mucosa.  Place the swab into the tube, breaking the swab off so that it remains in the tube.  Cover and send to the lab with appropriate paperwork. 

 

Warning:  Do NOT pre-wet swab in Cobas® PCR media before collection.

 

Ordering CT/NG Testing

Please use a DCDS Gyn Cytology form available through Dahl-Chase Diagnostic Services.