Dahl Chase Folders

BRAF Mutation Assay

Reference Number: AA-00312

BRAF V600 Mutation Detection


Clinical Significance:

BRAF V600 mutation analysis is useful in determining patient eligibility for targeted EGFR inhibitor therapy for metastatic colorectal carcinoma and for Lynch syndrome reflex testing (in colorectal carcinomas).


Specimen Requirements and Collection:

Formalin fixed tissue containing a sufficient amount of tumor (generally at least several mm of tumor tissue submitted in the tissue block).  The BRAF V600 test utilized by our laboratory will detect mutations that constitute at least 10% of the DNA sample population.



DNA isolation and extraction, library prep, and next-generation sequencing (NGS) for hotspot mutations BRAF exon 15



Molecular Pathology requisition form



Send formalin-fixed, paraffin-embedded (FFPE) tissue and cell block containing tumor at room temperature.  Also acceptable 10-unstained, 4-5 micron slides with 1 post H&E.  Please include a surgical pathology report


Unacceptable specimen:

Specimens fixed in alternative fixatives or metal fixatives (ex. B-plus).  Decalcified specimens.


Reference Range:

BRAF V600 mutation not detected = negative result

BRAF V600 mutation detected = positive result


CPT codes:

88381, 81210, G0452


Test reported:

Results are reported within 7-10 days