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Dahl Chase Folders

DNA Ploidy and Cell Cycle Analysis

Reference Number: AA-00235


SECTION FIVE

Dahl-Chase Diagnostic Services

FLOW CYTOMETRY

DNA PLOIDY AND CELL CYCLE ANALYSIS

 

Clinical Significance:

 

Nuclear DNA content can be measured and analyzed by flow cytometry to determine the DNA ploidy of the sample. Normal cells have a diploid DNA content while many tumors show cells with an abnormal DNA content (aneuploidy). Cell cycle analysis may be used to determine the proliferative activity of a neoplasm. Aneuploidy is considered a bad prognostic factor in many solid tumors but has positive implication regarding treatment in acute lymphoblastic leukemia, neuroblastoma and medulloblastoma. DNA Ploidy is also a helpful tool in the diagnosis of molar pregnancies.

 

Who should be tested?

Patients with suspected molar pregnancies, some solid tumors and leukemias

 

Specimens:

5 x 50 micron paraffin embedded sections

Bone marrow in Na Heparin

Peripheral blood in EDTA

Lymph node or other biopsy in Flow Cytometry medium

Body fluids in RPMI medium

 

Forms:

Dahl-Chase Flow Cytometry requisition form along with demographic sheet

 

Transport:

Sections from paraffin blocks may be ordered through the pathologist

Fresh Specimens need to be delivered and analyzed within 24-48 hours.

Uniship courier pick-up available.

 

CPT code:

88182

 

Test performed:

Monday through Saturday.

 

Test reported:

Faxed or mailed within 24 hours after sample is received.