Leukemia and Lymphoma Evaluation
Clinical Significance
Leukemia and lymphoma populations display
characteristic patterns of antigen expression (CD antigens) on the cell surface
or in the cytoplasm. Flow cytometric analysis provides increased sensitivity and
facilitates identification and proper classification of the hematopoietic
neoplasm. This information plays an important role in diagnosis and treatment
decisions.
Who should be tested:
Patients with suspected leukemia or
lymphoproliferative disorders
Antibody Panels:
These are designed based on the suspected diagnosis and may include any of the
following: CD2,
CD3, CD4, CD5, CD7, CD8, CD10, CD11b, CD11c, CD13, CD14, CD15, CD16, CD19,
CD20, CD22, CD23, CD24, CD25, CD33, CD34, CD38, CD45, CD56, CD57, CD64, CD79a, CD103,
CD117, CD123, CD138, CD200, CD235a, HLA-DR, kappa, lambda, myeloperoxidase, and
TdT
Specimens:
2-3 ml peripheral blood in EDTA (20°C)
1-2 ml bone marrow in Na Heparin (20°C)
Lymph node or other biopsy in RPMI Flow
Cytometry Medium (4°C)
Body fluid in any clean leak-proof container
(4°C)
CSF in RPMI Flow Cytometry Medium (4°C)
Fine needle aspiration (FNA) in RPMI Flow
Cytometry Medium (4°C)
Forms:
Dahl-Chase Flow Cytometry requisition form
along with demographic sheet
Copy of most recent CBC (for bone marrows and
peripheral blood samples)
Transport: Please call the flow cytometry lab at
941-8282 to ensure timely transport&delivery
Specimens need to be delivered and analyzed
within 24-36 hours.
Uniship courier pick-up available
Antibody Panels / CPT
codes:
88184 (first antibody, CD45)
88185 X the number of antibodies used for
disease-specific panel
88187 (2-8 antibodies), 88188 (9-15
antibodies) or 88189 (16 antibodies or more)
Test performed:
Monday through Saturday. Stat analysis on weekends
available per on-call pathologist
Test reported:
Faxed or mailed within 24 hours after sample
is received. Verbal can be given the same day that the specimen is received if
requested.
