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Dahl Chase Folders

Leukemia and Lymphoma Evaluations

Reference Number: AA-00234


SECTION FIVE

Dahl-Chase Diagnostic Services

FLOW CYTOMETRY

LEUKEMIA / LYMPHOMA EVALUATION

 

Clinical Significance:

 

Leukemia and lymphoma populations display characteristic patterns of antigen expression (CD antigens) on the cell surface or in the cytoplasm. Flow cytometric analysis provides increased sensitivity and facilitates identification and proper classification of the hematopoietic neoplasm. This information plays an important role in diagnosis and treatment decisions.

 

Who should be tested:

Patients with suspected leukemia or lymphoproliferative disorders

 

Antibody Panels:

Markers used may include any combination of the following:

CD2, CD3, CD4, CD5, CD7, CD8, CD10, CD11b, CD11c, CD13, CD14, CD15, CD16, CD19, CD20, CD22, CD23, CD25, CD33, CD34, CD38, CD45, CD56, CD57, CD64, CD103, CD117, CD123, CD138, CD235a, HLA-DR, kappa, lambda, myeloperoxidase, and TdT

 

Specimens:

2-3 ml peripheral blood in EDTA (20°C)

1-2 ml bone marrow in Na Heparin (20°C)

Lymph node or other biopsy in RPMI Flow Cytometry Medium (4°C)

Body fluid in any clean leak-proof container (4°C)

CSF in RPMI Flow Cytometry Medium (4°C)

Fine needle aspiration (FNA) in RPMI Flow Cytometry Medium (4°C)

 

Forms:

Dahl-Chase Flow Cytometry requisition form along with demographic sheet

Copy of most recent CBC (for bone marrows and peripheral blood samples)

 

Transport: 

Specimens need to be delivered and analyzed within 24-36 hours.

Uniship courier pick-up available

 

Antibody Panels / CPT codes:

88184 (first antibody, CD45)

88185 X the number of antibodies used for disease-specific panel

88187 (2-8 antibodies), 88188 (9-15 antibodies) or 88189 (16 antibodies or more)

 

Test performed:

Monday through Saturday. Stat analysis on weekends available per on-call pathologist

 

Test reported:

Faxed or mailed within 24 hours after sample is received. Verbal can be given the same day that the specimen is received if requested.