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Paroxysmal Nocturnal Hemoglobinuria Analysis

Reference Number: AA-00231

Out of state clients use this requisition when sending PNH testing

Paroxysmal Nocturnal Hemoglobinuria (PNH) Requisition for Out of State Clients

 

SECTION FIVE

Dahl-Chase Diagnostic Services

FLOW CYTOMETRY

PAROXYSMAL NOCTURNAL HEMOGLOBINURIA (PNH)

 

Clinical Significance:

 Paroxysmal nocturnal hemoglobinuria (PNH) is a life threatening and debilitating disease.  Clinically, PNH is characterized by chronic intravascular hemolysis, bone marrow failure and life threatening thrombosis.  PNH evolves from a hematopoietic stem cell defect in which a somatic mutation of an x-linked gene (PIG-A) results in a partial or absolute deficiency of GPI-linked proteins.  Absent or markedly diminished expression of GPI-linked antigens is specific for all patients with PNH. 

 

Who should be tested:

Patients with aplastic anemia, myelodysplastic syndrome, bone marrow failure syndromes or unexplained hemolysis.

 

Antibody Panels:

RBC panel:    CD235a-CD59

WBC panel:   FLAER-CD24-CD15-CD64-CD14-CD45 (monocytes and granulocytes)

 

Specimens:

1-2 ml peripheral blood in EDTA or heparin (4 °C or RT). 

 

Forms:

Dahl-Chase Flow Cytometry requisition form along with demographic sheet

CBC results obtained from the same tube

 

Transport:

Specimens need to be delivered and analyzed within 24-48 hours

Flow Cytometry lab should be notified of coming specimen with shipment alert

UniShip courier pick-up available for in-state specimens

FedEx pick-up available for out-of-state specimens

 

CPT code:

88184

88185 x 5

88187

 

Test performed:

Monday through Saturday.

 

Test reported:

Faxed or mailed within 24 hours after sample is received.

 

References:  1.     Borowitz et al: Guidelines for the Diagnosis and Monitoring of PNH and Related Disorders, Clin Cytometry 2010, 211-230

                      2.     Sutherland et al: Practical guidelines for the high-sensitivity detection and monitoring of PNH clones by flow cytometry. Cytometry B Clin Cytom 2012; 82:195-208.

                      3.     http://www.pnhsource.com/physicians